Rock Lab

Tracking the beautiful movements in biology


UChicago
Fascin-actin bundle

At the University of Chicago

  1. Bryan DS, Stack M, Krysztofiak K, Cichoń U, Thomas DG, Surcel A, Schiffhauer ES, Beckett MA, Khodarev NN, Xue L, Poli EC, Pearson AT, Posner MC, Robinson DN, Rock RS*, Weichselbaum RR*. 4-Hydroxyacetophenone modulates the actomyosin cytoskeleton to reduce metastasis. Proc Natl Acad Sci U S A. 117(36):22423-22429 (2020). PubMed

  2. Santos A, Shauchuk Y, Cichoń U, Vavra KC, Rock RS. How actin tracks affect myosin motors. Adv Exp Med Biol. 1239:183-197 (2020). PubMed

  3. Trejo CS, Rock RS, Stark WM, Boocock MR, Rice PA. Snapshots of a molecular swivel in action. Nucleic Acids Res. 46(10):5286-5296 (2018). PubMed

  4. Zalisko BE, Chan C, Denic V, Rock RS, Keenan RJ. Tail-anchored protein insertion by a single Get1/2 heterodimer. Cell Rep. 20(10):2287-2293 (2017). PubMed

  5. French AR, Sosnick TR, Rock RS. Investigations of human myosin VI targeting using optogenetically controlled cargo loading. Proc Natl Acad Sci U S A. 114(9):E1607-E1616 (2017) PubMed

  6. Alcala DB, Haldeman BD, Brizendine RK, Krenc AK, Baker JE, Rock RS, Cremo CR. Myosin light chain kinase steady-state kinetics: comparison of smooth muscle myosin II and nonmuscle myosin IIB as substrates. Cell Biochem Funct. 34(7):469-474 (2016). PubMed

  7. Vavra KC, Xia Y, Rock RS. Competition between coiled-coil structures and the impact on myosin-10 bundle selection. Biophys J. 110(11):2517-27 (2016). PubMed

  8. Zimmermann D, Santos A, Kovar DR, Rock RS. Actin age orchestrates myosin-5 and myosin-6 run lengths. Curr Biol. 25(15):2057-62 (2015). PubMed

  9. Surcel A, Ng WP, West-Foyle H, Zhu Q, Ren Y, Avery LB, Krenc AK, Meyers DJ, Rock RS, Anders RA, Freel Meyers CL, Robinson DN. Pharmacological activation of myosin II paralogs to correct cell mechanics defects. Proc Natl Acad Sci U S A. 112(5):1428-33 (2015). PubMed

  10. Engelmann BW, Kim Y, Wang M, Peters B, Rock RS, Nash PD. The development and application of a quantitative peptide microarray based approach to protein interaction domain specificity space. Mol Cell Proteomics. 13(12):3647-62 (2014). PubMed

  11. Rock RS. Cytoskeletal motors: general principles. In: Lennarz WJ, Lane MD, ed. The Encyclopedia of Biological Chemistry: 2nd Edition. Vol. 3. Waltham, MA: Academic Press; p. 632–635 (2013).

  12. Rock RS. Molecular motors: a finicky myosin V chooses its own path. Curr Biol. 22(15):R606-8 (2012). PubMed

  13. Spudich JA, Rice SE, Rock RS, Purcell TJ, Warrick HM. The optical trapping dumbbell assay for nonprocessive motors or motors that turn around filaments. Cold Spring Harb Protoc. 2011(11):1372-4 (2011). PubMed

  14. Spudich JA, Rice SE, Rock RS, Purcell TJ, Warrick HM. Attachment of anti-GFP antibodies to microspheres for optical trapping experiments. Cold Spring Harb Protoc. 2011(11):1370-1 (2011). PubMed

  15. Spudich JA, Rice SE, Rock RS, Purcell TJ, Warrick HM. Optical traps to study properties of molecular motors. Cold Spring Harb Protoc. 2011(11):1305-18 (2011). PubMed

  16. Skau CT, Courson DS, Bestul AJ, Winkelman JD, Rock RS, Sirotkin V, Kovar DR. Actin filament bundling by fimbrin is important for endocytosis, cytokinesis, and polarization in fission yeast. J Biol Chem. 286(30):26964-77 (2011). PubMed

  17. Rock RS. Muscle and myosin. In: Roux B, ed. Molecular Machines Singapore: World Scientific Press; p. 99–114 (2011).

  18. Ricca BL, Rock RS. The stepping pattern of myosin X is adapted for processive motility on bundled actin. Biophys J. 99(6):1818-26 (2010). PubMed

  19. Norstrom MF, Smithback PA, Rock RS. Unconventional processive mechanics of non-muscle myosin IIB. J Biol Chem. 285(34):26326-34 (2010). PubMed

  20. Courson DS, Rock RS. Actin cross-link assembly and disassembly mechanics for alpha-actinin and fascin. J Biol Chem. 285(34):26350-7 (2010). PubMed

  21. Nagy S, Rock RS. A structured post-IQ domain governs selectivity of myosin X for fascin-actin bundles. J Biol Chem. 285(34):26608-17 (2010). PubMed

  22. Rock RS. Intracellular transport: force controls motor switching at filament junctions. Curr Biol. 20(12):R525-7 (2010). PubMed

  23. Brawley CM, Uysal S, Kossiakoff AA, Rock RS. Characterization of engineered actin binding proteins that control filament assembly and structure. PLoS One. 5(11):e13960 (2010). PubMed

  24. Huang J, Nagy SS, Koide A, Rock RS, Koide S. A peptide tag system for facile purification and single-molecule immobilization. Biochemistry. 48(50):11834-6 (2009). PubMed

  25. Rock RS. A new direction for titin pulling. Proc Natl Acad Sci U S A. 106(32):13149-50 (2009). PubMed

  26. Rizk SS, Luchniak A, Uysal S, Brawley CM, Rock RS, Kossiakoff AA. An engineered substance P variant for receptor-mediated delivery of synthetic antibodies into tumor cells. Proc Natl Acad Sci U S A. 106(27):11011-11015 (2009). PubMed

  27. Ren Y, Effler JC, Norstrom M, Luo T, Firtel RA, Iglesias PA, Rock RS, Robinson DN. Mechanosensing through cooperative interactions between myosin II and the actin crosslinker cortexillin I. Curr Biol. 19(17):1421-1428 (2009). PubMed

  28. Brawley CM, Rock RS. Unconventional myosin traffic in cells reveals a selective actin cytoskeleton. Proc Natl Acad Sci U S A. 106(24):9685-9690 (2009). PubMed

  29. Courson DS, Rock RS. Fast benchtop fabrication of laminar flow chambers for advanced microscopy techniques. PLoS One. 4(8):e6479 (2009). PubMed

  30. Rizvi SA, Courson DS, Keller VA, Rock RS, Kozmin SA. The dual mode of action of bistramide A entails severing of filamentous actin and covalent protein modification. Proc Natl Acad Sci U S A. 105(11):4088-92 (2008). PubMed

  31. Nagy S, Ricca BL, Norstrom MF, Courson DS, Brawley CM, Smithback PA, Rock RS. A myosin motor that selects bundled actin for motility. Proc Natl Acad Sci U S A. 105(28):9616-9620 (2008). PubMed

Earlier

  1. Spudich JA, Rice SE, Rock RS, Purcell TJ, Warrick HM. Optical traps to study properties of molecular motors. In: Ha T, Selvin PR, ed. Single Molecule Techniques: A Laboratory Manual Cold Spring Harbor, N. Y.: Cold Spring Harbor Laboratory Press; p. 279–296 (2007).

  2. Churchman LS, Ökten Z, Rock RS, Dawson JF, Spudich JA. Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time. Proc Natl Acad Sci U S A. 102(5):1419-23 (2005). PubMed

  3. Rock RS, Ramamurthy B, Dunn AR, Beccafico S, Rami BR, Morris C, Spink BJ, Franzini-Armstrong C, Spudich JA, Sweeney HL. A flexible domain is essential for the large step size and processivity of myosin VI. Mol Cell. 17(4):603-9 (2005). PubMed

  4. Ökten Z, Churchman LS, Rock RS, Spudich JA. Myosin VI walks hand-over-hand along actin. Nat Struct Mol Biol. 11(9):884-7 (2004). PubMed

  5. Rock RS, Hansen KC, Larsen RW, Chan SI. Rapid photochemical triggering of protein unfolding in a nondenaturing environment. Chemical Physics. 307(2-3):201–208 (2004).

  6. Rock RS, Purcell TJ, Spudich JA. The mechanics of unconventional myosins. In: Hackney DD, Tamanoi F, ed. The Enzymes: 3rd Edition. Vol. 23. San Diego, CA: Elsevier Academic Press; p. 55–87 (2003).

  7. Robinson DN, Ocon SS, Rock RS, Spudich JA. Dynacortin is a novel actin bundling protein that localizes to dynamic actin structures. J Biol Chem. 277(11):9088-95 (2002). PubMed

  8. Spudich JA, Rock RS. A crossbridge too far. Nat Cell Biol. 4(1):E8–10 (2002). PubMed

  9. Rock RS, Rice SE, Wells AL, Purcell TJ, Spudich JA, Sweeney HL. Myosin VI is a processive motor with a large step size. Proc Natl Acad Sci U S A. 98(24):13655-9 (2001). PubMed

  10. Murphy CT, Rock RS, Spudich JA. A myosin II mutation uncouples ATPase activity from motility and shortens step size. Nat Cell Biol. 3(3):311-5 (2001). PubMed

  11. Rock RS, Rief M, Mehta AD, Spudich JA. In vitro assays of processive myosin motors. Methods. 22(4):373-81 (2000).

  12. Rief M, Rock RS, Mehta AD, Mooseker MS, Cheney RE, Spudich JA. Myosin-V stepping kinetics: a molecular model for processivity. Proc Natl Acad Sci U S A. 97(17):9482-6 (2000). PubMed

  13. Hansen KC, Rock RS, Larsen RW, Chan SI. A method for photoinitiating protein folding in a nondenaturing environment. J. Am. Chem. Soc. 122:11567-11568 (2000).

  14. Mehta AD, Rock RS, Rief M, Spudich JA, Mooseker MS, Cheney RE. Myosin-V is a processive actin-based motor. Nature. 400(6744):590-3 (1999). PubMed

  15. Rock RS, Chan S. Preparation of a water-soluble cage based on 3,5-dimethoxybenzoin. J. Am. Chem. Soc. 120:10766-10767 (1998).

  16. Rock RS, Chan S. Synthesis And Photolysis Properties Of A Photolabile Linker Based On 3'-Methoxybenzoin. J Org. Chem. 61(4):1526-1529 (1996). (1996)

  17. Stowell MH, Rock RS, Rees DC, Chan SI. Efficient synthesis of photolabile alkoxy benzoin protecting groups. Tet. Lett. 37(3):307–310 (1996).

Preprints

  1. Santos A, Kalita J, Rock RS. Slow and steady wins the race: encounters of myosin-5 and myosin-6 on shared actin filaments. bioRxiv

  2. Krenc AK, Rokicka JJ, Rock RS. A FLIC motility assay reveals myosin-6 coordination limited by actin filament buckling. bioRxiv

Biological Sciences Division | Dept. of Biochemistry | Institute for Biophysical Dynamics
© Ron Rock. Last modified: September 13, 2020.